endothelial cell growth medium 2 supplement pack (PromoCell)

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PromoCell endothelial cell growth medium 2 supplement pack
Endothelial Cell Growth Medium 2 Supplement Pack, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/growth+medium+2+supplement+pack/Endothelial+Cell+Growth+Medium+2/pmc12644823-30-40-47
Average 97 stars, based on 195 article reviews

endothelial cell growth medium 2 supplement pack - by Bioz Stars, 2026-09

97/100 stars

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Article Title: Composite Polylactide/Polycaprolactone Foams with Hierarchical Porous Structure for Pre-Vascularized Tissue Engineering
Article Snippet: HUVECs were purchased from PromoCell (Heidelberg, Germany, Cat. No. C-12200-single donor) and expanded in endothelial cell growth medium 2 (EGM-2), which was prepared from endothelial cell basal medium 2 (EBM-2, PromoCell, Heidelberg, Germany, Cat. No. C-22111), supplemented with the growth medium 2 supplement pack (PromoCell, Heidelberg, Germany, Cat. No. C-39211) containing hydrocortisone, heparin, ascorbic acid, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), FGF-2, 2% of FBS, and also with 1% antibiotic antimycotic solution ( v / v , A5955, Sigma-Aldrich, Saint Louis, MO, USA).

Article Title: Composite Polylactide/Polycaprolactone Foams with Hierarchical Porous Structure for Pre-Vascularized Tissue Engineering
Article Snippet: Human Umbilical Vein Endothelial Cells (HUVECs) HUVECs were purchased from PromoCell (Heidelberg, Germany, Cat. No. C-12200single donor) and expanded in endothelial cell growth medium 2 (EGM-2), which was prepared from endothelial cell basal medium 2 (EBM-2, PromoCell, Heidelberg, Germany, Cat. No. C-22111), supplemented with the growth medium 2 supplement pack (PromoCell, Heidelberg, Germany, Cat. No. C-39211) containing hydrocortisone, heparin, ascorbic acid, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), FGF-2, 2% of FBS, and also with 1% antibiotic antimycotic solution (v/v, A5955, Sigma-Aldrich, Saint Louis, MO, USA).

Article Title: Argon plasma-modified bacterial nanocellulose: Cell-specific differences in the interaction with fibroblasts and endothelial cells
Article Snippet: Another cell type used in this study was HUVECs (CLS Cell Lines Service, Eppelheim, Germany; Cat. No. 300,605), cultivated in Endothelial Cell Growth Medium 2 (EGM2), prepared from Endothelial Cell Basal Medium 2 (PromoCell GmbH, Heidelberg, Germany; Cat. No. C-22,111), supplemented with 1 % of antibiotic-antimycotic solution (v/ v, Sigma-Aldrich, St. Louis, MO, USA; Cat. No. A5955) and the growth medium-2 supplement pack (PromoCell, Heidelberg, Germany; Cat. No. C-39,211).

Article Title: Argon plasma-modified bacterial nanocellulose: Cell-specific differences in the interaction with fibroblasts and endothelial cells
Article Snippet: Human umbilical vein endothelial cells (HUVECs) purchased from CLS Cell Lines Service (Eppelheim, Germany; Cat. No. 300,605) were cultivated in Endothelial Cell Growth Medium 2 (EGM2), which was prepared from Endothelial Cell Basal Medium 2 (PromoCell GmbH, Heidelberg, Germany; Cat. No. C-22,111), supplemented with 1 % of antibiotic-antimycotic solution (v/v, Sigma-Aldrich, St. Louis, MO, USA; Cat. No. A5955) and the growth medium-2 supplement pack (PromoCell, Heidelberg, Germany; Cat. No. C-39,211) containing hydrocortisone, heparin, ascorbic acid, epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), fibroblast growth factor-2 (FGF-2) and 2 % of FBS.

Article Title: Cross-linked poly(alkylene citrates) with L-glutathione for vascular tissue engineering: structure-property relationships and cell-type dependent response to oxidative stress.
Article Snippet: Thus, in addition to ASCs, the following cell models were used: Normal human neonatal dermal fibroblasts (NHDFs), purchased from Lonza (Basel, Switzerland, Cat. No. CC-2509) and seeded at a density of 2.0 104 cells per sample (approximately 10 000 cells cm 2) in DMEM containing 10% FBS and gentamicin (40 mg mL 1); Human umbilical vein endothelial cells (HUVECs), purchased from Lonza (Basel, Switzerland, Cat. No. C2517A), were seeded at a density of 2.0 104 cells per sample (approximately 10 000 cells cm 2) in EGM2 basal medium (PromoCell, Heidelberg, Germany, Cat. No. C-22211) supplemented with hydrocortisone, heparin, ascorbic acid and 2% FBS from the growth medium supplement package 2 (PromoCell, Heidelberg, Germany, Cat. No. C-39211) and antibiotic & antimycotics (AbAm; Sigma-Aldrich, Merck, Darmstadt, Germany, 1 : 100; Cat. No. A5955-100ML); and Human umbilical artery smooth muscle cells (SMCs), purchased from PromoCell (Heidelberg, Germany, Cat. No. C-12500) and seeded at a density of 3.0 104 cells per sample (approximately 15 500 cells cm 2) in SMCGM-2 basal medium (PromoCell, Heidelberg, Germany, Cat. No. C-22262) supplemented with epidermal growth factor, basic fibroblast growth factor, insulin and 5% FBS in the growth medium 2 supplement pack (PromoCell, Heidelberg, Germany, Cat. No. C-39262) and with AbAm (Sigma-Aldrich, Merck, Darmstadt, Germany, 1 : 100; Cat. No. A5955-100ML).

Cell Culture:

Article Title: DLC1 promotes mechanotransductive feedback for YAP via RhoGAP-mediated focal adhesion turnover.
Article Snippet: Secondary antibodies coupled to horseradish peroxidase (HRP) were obtained from Bio-Rad (diluted 1:1000 for WB). .. Cell Culture Pooled primary Human Umbilical Vein Endothelial Cells (HUVECs) from different donors (Lonza) were cultured in Endothelial Cell Growth Medium 2 culture medium supplemented with the Growth Medium 2 Supplement Pack (PromoCell) on gelatin coated tissue flasks. .. HEK293T cells (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with L-glutamine and supplemented with 10% FCS and 1% pen/strep.

Article Title: Two-pore channel protein 2-mediated calcium release promotes angiopoietin 2 secretion by regulating Rab46-dependent Weibel-Palade body trafficking
Article Snippet: .. Pooled human umbilical vein endothelial cells (HUVECs: PromoCell) cultured in endothelial cell basal medium (EBM-2) supplemented with endothelial growth medium-2 supplement pack (EGM-2, PromoCell). ..

Article Title: NAADP-mediated calcium release promotes angiopoietin 2 secretion by regulating Rab46-dependent Weibel-Palade body trafficking.
Article Snippet: .. Pooled human umbilical vein endothelial cells (HUVECs: PromoCell) cultured in endothelial cell basal medium (EBM-2) supplemented with endothelial growth medium-2 supplement pack (EGM-2: PromoCell). ..

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